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Tryptone 17 56
2 at 25°C Suspend 20
Pepsin will cut the peptide chain anywhere there is a phenylalanine or leucine bond
CaCl2 is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of calcium or to replace this element
YT Top Agar Size:250g Tryptone 17 56Component g l Tryptone 8 Yeast extract 5 NaCl 5 Agar 7 Final pH 7. 0 0. 2 at 25C Suspend 25 gram powdered medium in 1 litre distilled water Store dry at room temperature.
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